谷氨酸棒桿菌

Corynebacterium glutamicum (Kinoshita et al.) Abe et al.

生產(chǎn)谷氨酸L-谷氨酸,，L-谷氨酸,，谷氨酸轉(zhuǎn)化宿主

菌落直徑為1-2mm，圓形,，邊緣整齊,，半透明，正面黃色,，反面黃色,，中間凸起，表面光滑,，質(zhì)地濕潤,，易挑起，不產(chǎn)色素,，G＋（藍(lán)紫色）,，桿菌，純度：純

蛋白胨：10.0,，牛肉粉：3.0,，氯化鈉：5.0，瓊脂：15.0,，pH值：7.3±0.1(25℃)

37℃,；18-24h；好氧,；

①準(zhǔn)備1支含有5~10mL液體培養(yǎng)基的試管和2個(gè)平板,；②安全柜中打開，用酒精燈灼燒頂部,，后迅速滴上無菌水使之破裂,，隨后用鑷子將其敲碎；③吸取0.5mL液體培養(yǎng)基打入凍干管,，充分溶解后重新打回液體試管中,，混勻；④吸取0.2mL菌懸液打入平板中,，涂布均勻,，重復(fù)兩次獲得兩個(gè)平板；⑤將液體試管和平板全部置于上述培養(yǎng)條件下培養(yǎng),，菌種長出即可使用,。

2-8℃

凍干粉；斜面,；菌液,；平板

‖Genome Sequenced Strain│Corynebacterium glutamicum ATCC 13032 DNA, complete genome.‖U.S. Patent Number│3,002,889‖Cross References│Nucleotide (GenBank) Y09578 C.glutamicum leuB gene.
Nucleotide (GenBank) M16175 C.glutamicum 5S rRNA.
Nucleotide (GenBank) X93513 C.glutamicum amt gene.
Nucleotide (GenBank) X93514 C.glutamicum betP gene.
Nucleotide (GenBank) X95649 C.glutamicum ORF4 gene.
Nucleotide (GenBank) Y09163 C.glutamicum putP gene.
Nucleotide (GenBank) X81379 C.glutamicum dapE gene and orf2.
Nucleotide (GenBank) X85965 C.glutamicum ORF3 and aroP gene.
Nucleotide (GenBank) A78796 Sequence 1 from Patent EP0563527.
Nucleotide (GenBank) A78797 Sequence 2 from Patent EP0563527.
Nucleotide (GenBank) A78798 Sequence 3 from Patent EP0563527.
Nucleotide (GenBank) X89850 C.glutamicum DNA for attB region.
Nucleotide (GenBank) X78491 C.glutamicum (ATCC 13032) aceB gene.
Nucleotide (GenBank) X89084 C.glutamicum pta gene and ackA gene.
Nucleotide (GenBank) Y09548 Corynebacterium glutamicum pyc gene.
Nucleotide (GenBank) X96580 C.glutamicum panB, panC & xylB genes.
Nucleotide (GenBank) Y13221 Corynebacterium glutamicum glnA gene.
Nucleotide (GenBank) AJ238250 Corynebacterium glutamicum ndh gene.
Nucleotide (GenBank) Y17592 Corynebacterium glutamicum accDA gene.
Nucleotide (GenBank) AJ012293 Corynebacterium glutamicum ilvD gene.
Nucleotide (GenBank) X90356 C.glutamicum DNA for promoter fragment F1.
Nucleotide (GenBank) X90357 C.glutamicum DNA for promoter fragment F2.
Nucleotide (GenBank) X90358 C.glutamicum DNA for promoter fragment F10.
Nucleotide (GenBank) X90359 C.glutamicum DNA for promoter fragment F13.
Nucleotide (GenBank) X90360 C.glutamicum DNA for promoter fragment F22.
Nucleotide (GenBank) X90361 C.glutamicum DNA for promoter fragment F34.
Nucleotide (GenBank) X90362 C.glutamicum DNA for promoter fragment F37.
Nucleotide (GenBank) X90363 C.glutamicum DNA for promoter fragment F45.
Nucleotide (GenBank) X90364 C.glutamicum DNA for promoter fragment F64.
Nucleotide (GenBank) X90365 C.glutamicum DNA for promoter fragment F75.
Nucleotide (GenBank) X60312 C.glutamicum lysI gene for L-lysine permease.
Nucleotide (GenBank) X81191 C.glutamicum gluA, gluB, gluC and gluD genes.
Nucleotide (GenBank) X75504 C.glutamicum aceA gene and thiX genes (partial).
Nucleotide (GenBank) Y16642 Corynebacterium glutamicum lpd gene, complete CDS.
Nucleotide (GenBank) X70959 C.glutamicum gene leuA for isopropylmalate synthase.
Nucleotide (GenBank) X86157 C.glutamicum argC, argJ, argB, argD, and argF genes.
Nucleotide (GenBank) AJ004934 Corynebacterium glutamicum dapD gene, complete CDS.
Nucleotide (GenBank) X67737 C.glutamicum dapB gene for dihydrodipicolinate reductase.
Nucleotide (GenBank) X71489 C.glutamicum icd gene for monomeric isocitrate dehydrogenase.
Nucleotide (GenBank) M25819 C.glutamicum phosphoenolpyruvate carboxylase gene, complete cds.
Nucleotide (GenBank) X59404 Corynebacterium glutamicum, gdh gen for glutamate dehydrogenase.
Nucleotide (GenBank) U31281 Corynebacterium glutamicum biotin synthase (bioB) gene, complete cds.
Nucleotide (GenBank) E04376 DNA sequence encoding corynebacterium glutamicum isocitric acid lyase.
Nucleotide (GenBank) AF189147 Corynebacterium glutamicum ISCg2 transposase (tnp) gene, complete cds.
Nucleotide (GenBank) AB055218 Corynebacterium glutamicum gene for superoxide dismutase, complete cds.
Nucleotide (GenBank) AJ306418 Corynebacterium glutamicum ctaE gene, qcrC gene, qcrA gene and qcrB gene.
Nucleotide (GenBank) L01508 Corynebacterium glutamicum threonine dehydratase (ilvA) gene, complete cds.
Nucleotide (GenBank) AF005635 Corynebacterium glutamicum glutamine synthetase (glnA) gene, complete cds.
Nucleotide (GenBank) AJ269506 Corynebacterium glutamicum pck gene for phosphoenolpyruvate carboxykinase.
Nucleotide (GenBank) U43535 Corynebacterium glutamicum multidrug resistance protein (cmr) gene, complete cds.
Nucleotide (GenBank) U43536 Corynebacterium glutamicum heat shock, ATP-binding protein (clpB) gene, complete cds.
Nucleotide (GenBank) AJ006985 Streptomyces glaucescens GLA.0 gene cluster for 5’-hydroxystreptomycin biosynthesis.
Nucleotide (GenBank) E04377 DNA sequence encoding corynebacterium glutamicum isocitric acid lyase N-terminal fragment.
Nucleotide (GenBank) AF112536 Corynebacterium glutamicum ribonucleotide reductase beta-chain (nrdF) gene, complete cds.
Nucleotide (GenBank) AJ300822 Corynebacterium glutamicum secE gene, nusG gene, rplK gene, rplA gene and partial pkwR gene.
Nucleotide (GenBank) AF116184 Corynebacterium glutamicum L-aspartate-alpha-decarboxylase precursor (panD) gene, complete cds.
Nucleotide (GenBank) U35023 Corynebacterium glutamicum thiosulfate sulfurtransferase (thtR) gene, partial cds, acyl CoA carboxylase (accBC) gene, complete cds.
Nucleotide (GenBank) AJ306417 Corynebacterium glutamicum partial nrdF gene for ribonucleotide reductase beta-subunit and ctaD gene for cytochrome aa3 oxidase subunit I.
Nucleotide (GenBank) L09232 Corynebacterium glutamicum acetohydroxy acid synthase (ilvB) and (ilvN) genes, and acetohydroxy acid isomeroreductase (ilvC) gene, complete cds.
Nucleotide (GenBank) AF112535 Corynebacterium glutamicum putative glutaredoxin NrdH (nrdH), NrdI (nrdI), and ribonucleotide reductase alpha-chain (nrdE) genes, complete cds.
Nucleotide (GenBank) M89931 Corynebacterium glutamicum beta C-S lyase (aecD) and branched-chain amino acid uptake carrier (brnQ) genes, complete cds, and hypothetical protein Yhbw (yhbw) gene, partial cds.
Nucleotide (GenBank) U13922 Corynebacterium glutamicum putative type II 5-cytosoine methyltransferase (cglIM) and putative type II restriction endonuclease (cglIR) and putative type I or type III restriction endonuclease (clgIIR) genes, complete cds.
Nucleotide (GenBank) BA000036 Corynebacterium glutamicum ATCC 13032 DNA, complete genome.‖描述│Genome sequenced strain
Lysine uptake and exchange