產(chǎn)品中文名稱:坎氏弧菌 產(chǎn)品英文名稱:Vibrio campbellii (Baumann et al.) Baumann et al. 參考用途:該培養(yǎng)物是保藏者開發(fā)的“群體感應(yīng)試劑盒”(ATCC MP-6)中包含的幾種菌株之一。該菌株具有基因型luxN::tn5Kan,。它是一種僅對(duì)AI-2自誘導(dǎo)物有反應(yīng)的突變傳感器菌株,。 形態(tài)特性:菌落直徑為0.5-1mm,圓形,,邊緣整齊,,不透明,正面白色,,中間凸起,,表面光滑,質(zhì)地濕潤,,易挑起,G-(紅),,桿菌 培養(yǎng)基:蛋白胨:5.0,,酵母膏:1.0,檸檬酸鐵:0.1,,溴化鉀:0.08,,NaCl:19.45,MgCl2:5.98,,Na2SO4:3.24,,CaCl2:1.8,KCl:0.55,,Na2CO3:0.16,,Na2NO3:0.0016,Na2HPO4:0.008,氯化鍶:0.034,,硼酸:0.022,,硅酸鈉:0.004,氟化鈉:0.0024,,瓊脂:15.0,,pH:7.4-7.8(25℃) 培養(yǎng)條件:30℃;18-24h,;好氧 培養(yǎng)方法:①準(zhǔn)備上述平板 1-2 塊,;②安全柜中打開,用酒精燈灼燒頂部,,后迅速滴上無菌水使之破裂,,隨后用鑷子將其敲碎;③吸取 0.5mL 無菌水打入凍干管,,充分溶解菌粉后,,打入平板中 200μL/個(gè),涂布均勻,;④將平板置于上述培養(yǎng)條件下培養(yǎng),,菌種長出即可使用。 存儲(chǔ)形式:2-8℃ 提供形式:凍干粉,;斜面,;菌液;平板 備注:‖Genotype│luxNtn5Kan‖Genotype│luxNtn5Kan‖F(xiàn)AQs│Alternative supplier of medium for ATCC item no. BAA-1117Our records show that this item also grows on MacConkey agar. Date Updated 11/4/2013‖描述│This is one of several strains (ATCC BAA-1116 through ATCC BAA-1121) included in a "quorum sensing reporter kit" developed by the depositor. Vibrio harveyi BAA-1116 (and its derived mutants in panel MP-6) has been reclassified as Vibrio campbellii based on microarray comparative genome hybridization and multilocus sequence analysis. RefLin B, et al. Comparative genomic analyses identify the Vibrio harveyi genome sequenced strains BAA-1116 and HY01 as Vibrio campbellii. Environ. Microbiol. Rep. 2(1): 81-89, 2010. PubMed: 20686623
產(chǎn)品中文名稱:坎氏弧菌
產(chǎn)品英文名稱:Vibrio campbellii (Baumann et al.) Baumann et al.
參考用途:該培養(yǎng)物是保藏者開發(fā)的“群體感應(yīng)試劑盒”(ATCC MP-6)中包含的幾種菌株之一。該菌株具有基因型luxN::tn5Kan,。它是一種僅對(duì)AI-2自誘導(dǎo)物有反應(yīng)的突變傳感器菌株,。
形態(tài)特性:菌落直徑為0.5-1mm,圓形,,邊緣整齊,,不透明,正面白色,,中間凸起,,表面光滑,質(zhì)地濕潤,,易挑起,G-(紅),,桿菌
培養(yǎng)基:蛋白胨:5.0,,酵母膏:1.0,檸檬酸鐵:0.1,,溴化鉀:0.08,,NaCl:19.45,MgCl2:5.98,,Na2SO4:3.24,,CaCl2:1.8,KCl:0.55,,Na2CO3:0.16,,Na2NO3:0.0016,Na2HPO4:0.008,氯化鍶:0.034,,硼酸:0.022,,硅酸鈉:0.004,氟化鈉:0.0024,,瓊脂:15.0,,pH:7.4-7.8(25℃)
培養(yǎng)條件:30℃;18-24h,;好氧
培養(yǎng)方法:①準(zhǔn)備上述平板 1-2 塊,;②安全柜中打開,用酒精燈灼燒頂部,,后迅速滴上無菌水使之破裂,,隨后用鑷子將其敲碎;③吸取 0.5mL 無菌水打入凍干管,,充分溶解菌粉后,,打入平板中 200μL/個(gè),涂布均勻,;④將平板置于上述培養(yǎng)條件下培養(yǎng),,菌種長出即可使用。
存儲(chǔ)形式:2-8℃
提供形式:凍干粉,;斜面,;菌液;平板
備注:‖Genotype│luxNtn5Kan‖Genotype│luxNtn5Kan‖F(xiàn)AQs│Alternative supplier of medium for ATCC item no. BAA-1117Our records show that this item also grows on MacConkey agar. Date Updated 11/4/2013‖描述│This is one of several strains (ATCC BAA-1116 through ATCC BAA-1121) included in a "quorum sensing reporter kit" developed by the depositor. Vibrio harveyi BAA-1116 (and its derived mutants in panel MP-6) has been reclassified as Vibrio campbellii based on microarray comparative genome hybridization and multilocus sequence analysis. RefLin B, et al. Comparative genomic analyses identify the Vibrio harveyi genome sequenced strains BAA-1116 and HY01 as Vibrio campbellii. Environ. Microbiol. Rep. 2(1): 81-89, 2010. PubMed: 20686623
以上信息僅供參考,,請以實(shí)物批次為準(zhǔn),!