產品中文名稱：人胚腎細胞
產品英文名稱：293[HEK-293]
參考用途：/
形態(tài)特性：上皮細胞樣,，多角形,，邊緣不規(guī)則，單層貼壁生長
培養(yǎng)基：EMEM完全培養(yǎng)基：90%EMEM+10%FBS
培養(yǎng)條件：37℃,；5%CO2+95%空氣,；
培養(yǎng)方法：復蘇步驟：①凍存管從液氮或-80℃冰箱中取出放入PE手套中，迅速沒入37℃水浴鍋，搖晃凍存管加速溶解,，以1min內全部溶解為宜,；②在超凈臺中將溶解好的細胞液加入到裝有9mL完全培養(yǎng)基的離心管內，1000-1200rpm離心5min,，棄去上清液,，用1-2mL完全培養(yǎng)基重懸細胞。③將細胞懸液加入到含有5-6mL完全培養(yǎng)基的T25瓶中,，放入培養(yǎng)箱培養(yǎng),。
細胞傳代：①將舊培養(yǎng)液吸除，PBS清洗兩遍后,，加入1-2mL胰酶（0.25%Trypsin+0.02%EDTA）,；②鏡下觀察消化情況，在細胞邊緣縮小,，貼壁松動時（可用吸管吸起些許胰酶輕輕吹打細胞層某處,，肉眼可見細胞層脫落，即消化完成,，否則繼續(xù)消化）,，直接吸掉胰酶，加入5-6mL完全培養(yǎng)基,，輕輕吹打細胞層,，把細胞層吹落，吹散,。③將細胞懸液按1:2比例分到新的T25瓶中,，添加適當的完全培養(yǎng)基，把細胞懸液打勻,，于培養(yǎng)箱中培養(yǎng),。④注意培養(yǎng)基pH值變化和細胞密度，定期換液（每周2-3次）,，待細胞密度達到80%-90%時,，重復傳代操作或者凍存。
存儲形式：液氮
提供形式：凍存管,；T25培養(yǎng)瓶
備注：‖Age│fetus‖Karyotype│This is a hypotriploid human cell line. The modal chromosome number was 64, occurring in 30% of cells. The rate of cells with higher ploidies was 4.2 %. The der(1)t(1;15) (q42;q13), der(19)t(3;19) (q12;q13), der(12)t(8;12) (q22;p13), and four other marker chromosomes were common to most cells. Five other markers occurred in some cells only. The marker der(1) and M8 (or Xq+) were often paired. There were four copies of N17 and N22. Noticeably in addition to three copies of X chromosomes, there were paired Xq+, and a single Xp+ in most cells.‖Receptor Expression│vitronectin, expressed‖Tumorigenic│YES‖Effects│Yes, forms tumors in nude mice‖STR Profile│Amelogenin X
CSF1PO 11,12
D13S317 12,14
D16S539 9,13
D5S818 8,9
D7S820 11,12
THO1 7,9.3
TPOX 11
vWA 16,19‖FAQ’s│CRL-1573 culture conditions and cell densityDate Updated 4/7/2014‖Restrictions│ This material is sold by ATCC for research use only. Please direct any commercial license inquiries to AdVec Inc., C/O Frank L. Graham, Piazza delle 5 Scole 23, Rome 00186, ITALY. Email [email protected]‖描述│Although an earlier report suggested that the cells contained Adenovirus 5 DNA from both the right and left ends of the viral genome [RF32764], it is now clear that only left end sequences are present.
The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit.
The Ad5 insert was cloned and sequenced, and it was determined that a colinear seqment from nts 1 to 4344 is integrated into chromosome 19 (19q13.2).