產(chǎn)品中文名稱：人B淋巴瘤細(xì)胞
產(chǎn)品英文名稱：Farage
參考用途：/
形態(tài)特性：淋巴母細(xì)胞樣，圓形
培養(yǎng)基：90%RPMI-1640+10%FBS
培養(yǎng)條件：37℃；5%CO2+95%空氣；
培養(yǎng)方法：復(fù)蘇步驟：①凍存管從液氮或-80℃冰箱中取出放入PE手套中，迅速沒入37℃水浴鍋，搖晃凍存管加速溶解，以1min內(nèi)全部溶解為宜；②在超凈臺中將溶解好的細(xì)胞液加入到裝有9mL完全培養(yǎng)基的離心管內(nèi)，1000-1200rpm離心3-5min，棄去上清液，用1-2mL完全培養(yǎng)基重懸細(xì)胞。③然后將細(xì)胞懸液加入到含有6-7mL完全培養(yǎng)基的T25瓶中，放入培養(yǎng)箱培養(yǎng)。培養(yǎng)瓶建議豎著培養(yǎng)。
細(xì)胞傳代：①離心法：收集細(xì)胞，1000rpm離心5min，棄去上清液，補加1-2mL培養(yǎng)液后吹勻，將細(xì)胞懸液按1：2的比例分到新的含8mL培養(yǎng)基的T25瓶中。②半量換液法：可選擇半量換液方式；請將上清培養(yǎng)基輕輕吸走一半，將剩余留有細(xì)胞沉淀的培養(yǎng)基重懸混勻，將細(xì)胞懸液按1：2的比例分到新的含8mL培養(yǎng)基的T25瓶中。③注意培養(yǎng)基pH值變化和細(xì)胞密度，定期換液（每周2-3次），待細(xì)胞密度達(dá)到大于2×106個/mL時，重復(fù)傳代操作或者凍存。
存儲形式：液氮
提供形式：凍存管；T25培養(yǎng)瓶
備注：‖Age│adult‖Gender│female‖Ethnicity│White‖Karyotype│trisomy of chromosome 11 RefGabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin’s lymphoma cell lines. Eur. J. Immunol. 63 180-191, 1999. PubMed 10485273‖Derivation│The Farage cell line was adapted to culture in 1990 from a lymph node biopsy of a patient with diffuse large cell non-Hodgkin’s lymphoma (DLCL).‖Clinical Data│adult
female
White‖Antigen Expression│CD10 +/-; CD11a + (LFA-1); CD19 +; CD20 +; CD21 +; CD22 +; CD23 +; CD29 (VLA-4) +; CD38 +; CD39 +; CD40 +; CD44 +; CD54 + (ICAM-1); CD58 + (LFA-3); CD23 -; HLA DR +‖STR Profile│Amelogenin X
CSF1PO 11,12
D13S317 11,13
D16S539 11,12
D5S818 12
D7S820 12
THO1 8,9
TPOX 9
vWA 14,15‖Population Doubling Time│24 to 36 hrs‖Year of Origin│1990‖描述│The cells do not express surface or cytoplasmic immunoglobulin. Exposure to IL-4 augmented the concentrations of CD23, CD54, and CD58 but diminished the expression of CD21, CD22, and CD38. Incubation with IL-4 for 6 to 8 days led to increased expression of CD11a, CD39, CD40, and to disappearance of CD21 and CD38. Exposure of Farage cells to phorbol 12-myristate 13-acetate (PMA) down-regulated CD21 and CD23 expression. They do not express the terminal deoxynucleotydyl transferase gene (TdT), nor the recombination activating genes RAG-1 and RAG-2, known as markers of the pre-B cell stage. These results show that Farage represents a mature B-cell rather than a pre-B cell. The cells are positive for Epstein-Barr virus (EBV).
mature B cell